bbin宝盈集团官网

Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids

João Pedro Alves-Lopes, Frederick C. K. Wong, M. Azim Surani

Published: 2024-02-01 DOI: 10.1038/s41596-023-00945-1

primordial germ cell-like cells

hindgut organoids

co-culture system

gametogenesis

infertility

AI 解读

浏览 2550

page-1

page-2

page-3

page-4

page-5

page-6

page-7

page-8

page-9

page-10

page-11

page-12

page-13

page-14

page-15

page-16

page-17

page-18

page-19

page-20

page-21

page-22

page-23

page-24

page-25

page-26

page-27

page-28

page-29

page-30

page-31

page-32

page-33

page-34

page-35

page-36

page-37

page-38

page-39

page-40

推荐阅读

Nature Protocols

Protocols IO

Current Protocols

Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Genetic circuit design automation with Cello 2.0

Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

A practical guide to scanning light-field microscopy with digital adaptive optics

Effective high-throughput isolation of fully human antibodies targeting infectious pathogens

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

MOF-derived nanoporous carbons with diverse tunable nanoarchitectures

Tutorial: a statistical genetics guide to identifying HLA alleles driving complex disease

查看全部

Sections

Figures

References

Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Reporting summary
Acknowledgements

Tang, W. W. et al. A unique gene regulatory network resets the human germline epigenome for development. Cell 161, 1453–1467 (2015).
Gruhn, W. H. et al. Epigenetic resetting in the human germ line entails histone modification remodeling. Sci. Adv. 9, eade1257 (2023).
Ramakrishna, N. B., Murison, K., Miska, E. A. & Leitch, H. G. Epigenetic regulation during primordial germ cell development and differentiation. seks. Dev. 15, 411–431 (2021).
Garcia-Alonso, L. et al. Single-cell roadmap of human gonadal development. Nature 607, 540–547 (2022).
Tang, W. W. C., Kobayashi, T., Irie, N., Dietmann, S. & Surani, M. A. Specification and epigenetic programming of the human germ line. Nat. Rev. Genet. 17, 585–600 (2016).
Hargy, J. & Sasaki, K. The developmental dynamics of the human male germline. Development 150, dev202046 (2023).
Sasaki, K. et al. Robust in vitro induction of human germ cell fate from pluripotent stem cells. Cell Stem Cell 17, 178–194 (2015).
Kobayashi, T. et al. Principles of early human development and germ cell program from conserved model systems. Nature 546, 416–420 (2017).
Irie, N. et al. SOX17 is a critical specifier of human primordial germ cell fate. Cell 160, 253–268 (2015).
Kojima, Y. et al. Evolutionarily distinctive transcriptional and signaling programs drive human germ cell lineage specification from pluripotent stem cells. Cell Stem Cell 21, 517–532.e5 (2017).
Kojima, Y. et al. GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program. Life Sci. Alliance 4, e202000974 (2021).
Tang, W. W. C. et al. Sequential enhancer state remodelling defines human germline competence and specification. Nat. Cell Biol. 24, 448–460 (2022).
Sybirna, A. et al. A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons. Nat. Commun. 11, 1282 (2020).
Irie, N. et al. DMRT1 regulates human germline commitment. Nat. Cell Biol. 25, 1439–1452 (2023).
Yu, L. et al. Derivation of intermediate pluripotent stem cells amenable to primordial germ cell specification. Cell Stem Cell 28, 550–567.e12 (2021).
Kinoshita, M. et al. Capture of mouse and human stem cells with features of formative pluripotency. Cell Stem Cell 28, 453–471.e8 (2021).
Yamashiro, C. et al. Generation of human oogonia from induced pluripotent stem cells in vitro. Science 362, 356–360 (2018).
Yamashiro, C., Sasaki, K., Yokobayashi, S., Kojima, Y. & Saitou, M. Generation of human oogonia from induced pluripotent stem cells in culture. Nat. Protoc. 15, 1560–1583 (2020).
Hwang, Y. S. et al. Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells. Nat. Commun. 11, 5656 (2020).
Castillo-Venzor, A. et al. Origin and segregation of the human germline. Life Sci. Alliance 6, e202201706 (2023).
Guo, G. et al. Epigenetic resetting of human pluripotency. Development 144, 2748–2763 (2017).
Bayerl, J. et al. Principles of signaling pathway modulation for enhancing human naive pluripotency induction. Cell Stem Cell 28, 1549–1565.e12 (2021).
Rostovskaya, M., Stirparo, G. G. & Smith, A. Capacitation of human naïve pluripotent stem cells for multi-lineage differentiation. Development 146, dev172916 (2019).
Alves-Lopes, J. P. et al. Specification of human germ cell fate with enhanced progression capability supported by hindgut organoids. Cell Rep. 42, 111907 (2023).
Li, L. et al. Single-cell RNA-seq analysis maps development of human germline cells and gonadal niche interactions. Cell Stem Cell 20, 858–873.e4 (2017).
Spence, J. R. et al. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature 470, 105–109 (2011).
McCracken, K. W., Howell, J. C., Wells, J. M. & Spence, J. R. Generating human intestinal tissue from pluripotent stem cells in vitro. Nat. Protoc. 6, 1920–1928 (2011).
Chen, D. et al. Germline competency of human embryonic stem cells depends on eomesodermin. Biol. Reprod. 97, 850–861 (2017).

关键词

primordial germ cell-like cells

hindgut organoids

co-culture system

gametogenesis

infertility

扫码咨询