Overall protocol for top-down LC-MS/MS of human heart tissue
Jeannie Camarillo, Bryon Drown, Neil Kelleher
Published: 2022-03-02 DOI: 10.17504/protocols.io.b5tjq6kn
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Abstract
Overview description of the process for acquiring top-down LC-MS/MS data on human heart tissue
Steps
Tissue Collection
1.
Heart tissue was provided by University of Washington. Tissue was collected and prepared according to the following:
Protocol for Tissue Collection from Organ Procurement Organization
Sample Preparation
2.
Perform LC based Proteomics on adjacent tissue sections:
Tissue Homogenization and GELFrEE for Top-Down Proteomics
Data Acquisition
3.
Untargeted Top-down Proteomics by LC-MS/MS on Lumos
4.
Top-down proteomics data analysis on the NRTDP TDPortal
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