TEM
Alexander Röntgen
Published: 2023-11-02 DOI: 10.17504/protocols.io.q26g7pd79gwz/v1
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Abstract
TEM protocol.
Steps
Preparation
1.
Recover samples by pipetting out of aggregation plate
2.
Glow-discharge grids (3-mm 300-mesh (EM Resolutions Ltd))
Sample spotting and staining
3.
Take up grid with tweezers
4.
Spot 2.3 µL sample on grid
5.
Stain sample with 2.3 µL 2% uranyl acetate for 40 s at room temperature
Imaging
6.
Image grids on an electron microscope (FEI Tecnai G2 transmission electron microscope)
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