Take excisional biopsy of human skin in abdomen or scalp

After excisional biopsy from a operating room (OR), place the skin tissue into a tissue container prefilled with buffered formalin for 12-24 hours at room temperature (RT)

Discard formalin, rinse with PBS, refill PBS, and leave the tissue in PBS for 1 minute

Take tissue out on a large Petri dish and measure the tissue size

Label a slotted cassette with a pencil with tissue ID and place the tissue in the cassette

Put cassette with the tissue back into the tissue container filled with PBS

Dehydrate tissue by submerging cassettes into ethanol at increasing concentrations from 70% to 99%.

Embed cassette paraffin at 60 deg C and store at room temperature

Section tissue and place on water bath to expand

Place expanded tissue section on a Superfrost Plus microscope slide

Stain with Hematoxylin and Eosin

Place coverslip